Internet Archive's in-browser bookreader "theater" requires JavaScript to be enabled. It appears your browser does not have it turned on. Please see your browser settings for this feature.

How can integrated morphotaxonomy- and metabarcoding-based diatom assemblage analyses best contribute to the ecological assessment of streams?

by: Pissaridou, Panayiota; Cantonati, Marco; Bouchez, Agnes; Tziortzis, Iakovos; Dörflinger, Gerald; Vasquez, Marlen I.

Publication date: 2021-8-25
Usage: Attribution 4.0 International
Topics: DNA metabarcoding, morpho-taxonomy, river biomonitoring, Cyprus rivers
Publisher: Pensoft Publishers
Collection: biodiversity
Contributor: Pensoft Publishers
Language: English
Rights: https://biodiversitylibrary.org/permissions
Rights-holder: Copyright held by individual article author(s).
Volume: 5
Item Size: 19.9M

Abstract: Environmental conditions, such as nutrient concentrations, salinity, elevation etc., shape diatom assemblages of periphytic biofilms. These assemblages respond rapidly to environmental changes, a fact which makes diatoms valuable bioindicators. Hence, freshwater biomonitoring programmes currently use diatom indices (e.g. EU Water Framework Directive - WFD). To date, microscopy-based assessments require high taxonomic expertise for diatom identification at the species level. High-throughput technologies now provide cost-effective identification approaches that are promising, complementary or alternative tools for bioassessment. The suitability of the metabarcoding method is evaluated for the first time in the Cyprus streams WFD monitoring network, an eastern Mediterranean country with many endemic species and results are compared to the results acquired from the morphotaxonomic analysis. Morphotaxonomic identification was conducted microscopically, using the most updated taxonomic concepts, literature and online resources. At the same time, DNA metabarcoding involved the use of the rbcL 312 bp barcode, high-throughput sequencing and bioinformatic analysis. The ecological status was calculated using the IPS Index. Results show a positive correlation between morpho-taxonomic and molecular IPS scores. Discrepancies between the two methodologies are related to the limitations of both techniques. This study confirmed that Fistulifera saprophila can have a crucial role in key differences observed, as it negatively influences IPS scores and microscopy methods frequently overlook it. Importantly, gaps in the DNA barcoding reference databases lead to a positive overestimation in IPS scores. Overall, we conclude that DNA metabarcoding offsets the morphotaxonomic methodology for the ecological quality assessment of freshwaters.
Addeddate: 2025-05-08 20:08:38
Bhl_virtual_titleid: 210921
Bhl_virtual_volume: v.5 (2021)
Call number: 10_3897_mbmg_5_68438
Call-number: 10_3897_mbmg_5_68438
Foldoutcount: 0
Genre: article
Identifier: howcanintegrate5piss
Identifier-ark: ark:/13960/s2b69kvm67k
Identifier-bib: 10_3897_mbmg_5_68438
Identifier-doi: 10.3897/mbmg.5.68438
Ocr: tesseract 5.3.0-6-g76ae
Ocr_detected_lang: en
Ocr_detected_lang_conf: 1.0000
Ocr_detected_script: Latin
Ocr_detected_script_conf: 1.0000
Ocr_module_version: 0.0.21
Ocr_parameters: -l eng
Page_number_confidence: 77
Page_number_module_version: 1.0.5
Page_range: 1-10
Pages: 10
Pdf_degraded: invalid-jp2-headers
Pdf_module_version: 0.0.25
Possible copyright status: In copyright. Digitized with the permission of the rights holder.
Ppi: 300
Source: Metabarcoding and Metagenomics 5
Year: 2021